Hemagglutination with Newcastle Disease Virus (NDV)

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Abstract
Hemagglutination by Newcastle disease virus (NDV) is markedly affected by temp. At room temp. the reaction is difficult to read, especially with low dilutions of virus. At 4[degree]C the reaction is easily read and titration end-points are readily detd. At 4[degree]C NDV appears to be adsorbed more completely by and to elute less rapidly from chicken red cells than at room temp. Details of satisfactory methods for titration of virus and antibodies against it are presented. Both tests are carried out at 4[degree]C.