Binding and regulation of HIF‐1α by a subunit of the proteasome complex, PSMA7
- 30 May 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 498 (1) , 62-66
- https://doi.org/10.1016/s0014-5793(01)02499-1
Abstract
The hypoxia-inducible factor-1alpha (HIF-1alpha) is an important transcription factor for cellular responses to oxygen tension. It is rapidly degraded under normoxic conditions by the ubiquitin-dependent proteasome pathway. Here we report a critical role of the 20S proteasome subunit PSMA7 in HIF-1alpha regulation. PSMA7 was found to interact specifically with two subdomains of HIF-1alpha. PSMA7 inhibited the transactivation function of HIF-1alpha under both normoxic and hypoxia-mimicking conditions. In addition, we show that the PSMA7-mediated regulation of HIF-1alpha activity is associated with the proteasome pathway.Keywords
This publication has 31 references indexed in Scilit:
- Mechanism of regulation of the hypoxia-inducible factor-1alpha by the von Hippel-Lindau tumor suppressor proteinThe EMBO Journal, 2000
- Human Hepatitis B Virus X Protein Is a Possible Mediator of Hypoxia-Induced Angiogenesis in HepatocarcinogenesisBiochemical and Biophysical Research Communications, 2000
- Regulation of Mammalian O2Homeostasis by Hypoxia-Inducible Factor 1Annual Review of Cell and Developmental Biology, 1999
- THE UBIQUITIN SYSTEMAnnual Review of Biochemistry, 1998
- Transactivation and Inhibitory Domains of Hypoxia-inducible Factor 1αJournal of Biological Chemistry, 1997
- Activation of Hypoxia-inducible Factor-1; Definition of Regulatory Domains within the α SubunitJournal of Biological Chemistry, 1997
- Hypoxia Response Elements in the Aldolase A, Enolase 1, and Lactate Dehydrogenase A Gene Promoters Contain Essential Binding Sites for Hypoxia-inducible Factor 1Journal of Biological Chemistry, 1996
- Activation of Hypoxia-inducible Transcription Factor Depends Primarily upon Redox-sensitive Stabilization of Its α SubunitJournal of Biological Chemistry, 1996
- Interaction of HTLV-I Tax with the Human Proteasome: Implications for NF-κB InductionJAIDS Journal of Acquired Immune Deficiency Syndromes, 1996
- HBx protein of hepatitis B virus interacts with the C-terminal portion of a novel human proteasome alpha-subunitVirus Genes, 1995