INHIBITION BY PROPRANOLOL OF BILE ACID-STIMULATED AND PGE1-STIMULATED CAMP AND INTESTINAL SECRETION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 70  (2) , 129-135
Abstract
Colonic (3) and 3 ileal loops were prepared in 6 rabbits pretreated with propranolol (PR) 4 mg/kg i.v. and in 5 untreated rabbits. In random order, 1 ml of either deoxycholic acid (DCA) 6 mM, prostaglandin E1 (PGE1) 20 .mu.g/ml or saline was placed in each colonic loop and 1 ml of either cholera enterotoxin (CE) 10 .mu.g/ml PGE1 20 .mu.g/ml or saline wasplaced in each ileal loop. In untreated animals, DCA and PGE1 in the colon and CE and PGE1 in the ileum stimulated (P < 0.01) adenylate cyclase (AC) and net secretion. In the colon, PR abolished DCA stimulation of AC and net secretion and decreased PGE1-stimulated AC (P < 0.01) and net secretion. At the doses and times studied, colonic-AC and net secretion stimulated by PGE1 or DCA was distinguished from small bowel AC and net secretion stimulated by PGE1 or CE.