MODIFICATION OF ESCHERICHIA-COLI PHENYLALANYL-TRANSFER RNA-SYNTHETASE BY TRANSFER RNAPHE DERIVATIVES CARRYING REACTIVE GROUPS ON GUANOSINE RESIDUES
- 1 January 1980
- journal article
- research article
- Vol. 14 (3) , 419-425
Abstract
Photoreactive derivatives of tRNAPhe (E. coli) were synthesized by alkylation of the tRNAPhe with 4-(N-2-chloroethyl-N-methylamino)benzylamine and subsequent treatment with 1,4-dinitro-5-fluorophenylazide. The derivatives are active in binding with ribosomes and phenylalanyl-tRNA synthetase when the extent of modification is lower than 3 reactive groups/tRNAPhe molecule. Under irradiation, the derivatives modify exclusively the .beta.-subunit of the phenylalanyl-tRNA synthetase.This publication has 3 references indexed in Scilit:
- Functional covalent complex between elongation factor Tu and an analog of lysyl-tRNA.Proceedings of the National Academy of Sciences, 1978
- Sequence-specific crosslinking agents for nucleic acidsJournal of Molecular Biology, 1978
- The Isolation and Properties of Phenylalanyl Ribonucleic Acid Synthetase from Escherichia coli BJournal of Biological Chemistry, 1967