Biosynthesis of porphyrins and related macrocycles. Part 11. Studies on biosynthesis of the phytyl chain of chlorophyll a by use of carbon-13
- 1 January 1977
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Perkin Transactions 1
- No. 23,p. 2540-2545
- https://doi.org/10.1039/p19770002540
Abstract
A procedure is developed for the isolation on a small scale of pure phytol from cells of Euglena gracilis, and conditions are found for the separation of a crystalline phytyl carbamate from the corresponding derivative of geranylgeraniol Dark-grown cells of E. gracilis are shown to contain only negligible quantities of phytol, but on illumination they incorporate acetate efficiently into the phytyl chain of chlorophyll; the low dilution achieved allows experiments with [1 -13C] acetate. The eight sites of 13C enrichment in the resultant phytol are located by n.m.r. Presumably because of poor transport, the incorporation of mevalonate into phytol is very low.This publication has 10 references indexed in Scilit:
- Biosynthesis of porphyrins and related macrocycles. Part 10. Vitamin B12: biochemical derivation of cobyrinic acid from uroporphyrinogen III, studies with the corresponding ring c methyl hepta-carboxylic porphyrinogen, and proof of seven intact methyl transfersJournal of the Chemical Society, Perkin Transactions 1, 1977
- Biosynthesis of porphyrins and related macrocycles. Part VI. Nature of the rearrangement process leading to the natural type III porphyrinsJournal of the Chemical Society, Perkin Transactions 1, 1976
- The characterization and properties of castaprenol-11, -12 and -13 from the leaves of Aesculus hippocastanum (horse chestnut)Biochemical Journal, 1967
- Biosynthèse du phytol des chlorophylles et du squelette tétraterpénique des caroténoïdes dans les feuilles vertesPhytochemistry, 1966
- Incorporation of [14C]carbon dioxide and [2-14C]mevalonic acid into terpenoids of higher plants during chloroplast developmentBiochemical Journal, 1966
- Control by Iron of Chlorophyll Formation and Growth in Euglena gracilisPlant Physiology, 1964
- Studies of Chloroplast Development in Euglena. V. Pigment Biosynthesis, Photosynthetic Oxygen Evolution and Carbon Dioxide Fixation during Chloroplast DevelopmentPlant Physiology, 1964
- Spectrophotometric Determination of Chlorophylls and Pheophytins in Plant ExtractsAnalytical Chemistry, 1960
- A Pheophytin‐like Pigment in Dark‐Adapted Euglena gracilisThe Journal of Protozoology, 1959
- A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUESJournal of Biological Chemistry, 1957