Conformation of Macromolecules in the Gas Phase: Use of Matrix-Assisted Laser Desorption Methods in Ion Chromatography
- 10 March 1995
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 267 (5203) , 1483-1485
- https://doi.org/10.1126/science.267.5203.1483
Abstract
Conformational data for macromolecules in the gas phase have been obtained by the coupling of a matrix-assisted laser desorption ion source to an ion chromatograph. A series of polyethylene glycol (PEG) polymers "cationized" (converted to a cation) by sodium ions (Na+PEG9 to Na+PEG19) and a protonated neurotransmitter protein, bradykinin, were studied. Mobilities of Na+PEG9 to Na+PEG19 are reported. Detailed modeling of Na+PEG9 with molecular mechanics methods indicates that the lowest energy structure has the Na+ ion "solvated" by the polymer chain with seven oxygen atoms as nearest neighbors. The agreement between the model and experiment is within 1 percent for Na+PEG9, Na+PEG13, and Na+PEG17, giving strong support to both the method and the deduced structures. Similar agreement was obtained in initial studies that modeled experimental data for arginine-protonated bradykinin.Keywords
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