Stop-flow microperfusion of proximal tubules in rat kidney cortex slices

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Abstract
A method is described for the study of renal tubular fluid transport in vitro by the application of microperfusion techniques to proximal tubules in rat kidney cortex slices. A short column of colored low-viscosity silicone oil is infused into a surface tubule and split by the injection of isoosmotic saline. Sequential photographs show that reabsorption of this fluid proceeds exponentially. In control studies, where the bathing medium, a bicarbonate-saline providing 140 m[image] Na and 10 m[image] K, was oxygenated and maintained at 37 C, the mean reabsorptive half-time ,(tl/2) was 18.4[plus or minus]0.7 sec. Reduction of the temperature to 15 C prolonged t1/2 to 181[long dash]27 sec. When a K-free bathing medium was used, t1/2 was 36.3[plus or minus]2.7 sec. Under hypoxic conditions t1/2 was 36.6[plus or minus]5.5 sec.

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