Standardization of Techniques for Infecting Mice with Toxocara canis and Observations on the Normal Migration Routes of the Larvae

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Abstract
Mice were inoculated by stomach tube with 500 infective eggs of Toxocara canis and the resulting infections were measured in terms of percentages of inoculated larvae that were recovered by peptic digestion of the mouse tissues. The most uniform results were obtained by (1) silicon coating of all inner surfaces of containers and instruments used in the incubation and inoculation of eggs, (2) use of improved methods of estimating the number of eggs in the inoculum, (3) inoculation of the mice in the morning when their stomachs normally contained food, and (4) use of normal (non-decoated) eggs incubated for at least 4 weeks and not more than 8 weeks. Infections were increased slightly by morphine treatment of the mice before inoculation. Body weight had little influence on the infections. At 44 to 48 hours after inoculation 98% of the larvae were found in the liver. Although most of the larvae followed the somatic route of migration, some followed the tracheal route, returned to the intestine and re -entered the tissues during the 4th and 5th days after inoculation.

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