ANTIGEN—ANTIBODY CROSSED ELECTROPHORESIS OF WATER‐SOLUBLE RAT BRAIN ANTIGENS
- 1 December 1971
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 18 (12) , 2435-2440
- https://doi.org/10.1111/j.1471-4159.1971.tb00199.x
Abstract
—: A method of separation by antigen‐antibody crossed electrophoresis was applied to the study of water‐soluble rat brain antigens. Five different rat brain preparations were used as antigens for immunization. The extract from Triton X‐100 treated brains gave the best antibody response. An antiserum containing 27 precipitating antibodies was obtained. The preparation of antigens for immunoelectrophoresis was investigated. Treatment with demineralized water gave the highest number of antigen‐antibody precipitates. Treatment with detergents and sonication gave a higher total protein yield, but the number of precipitates was unaffected.Keywords
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