Isolation and characterization of tunicamycin resistant mutants from Chinese Hamster ovary cells
- 1 October 1979
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 101 (1) , 149-156
- https://doi.org/10.1002/jcp.1041010117
Abstract
Stable clones selected for resistance to tunicamycin (TM) have been isolated from Chinese Hamster Ovary (CHO) cells. The TMR phenotype is stable for more than nine months in the absence of the drug. The morphology of TMR mutant varies from epitheloid to abnormally elongate. The mutants do not display cross-resistance for ConA but are slightly cross-resistant to PHA. Biochemically labeled membrane proteins and glycoprotein of Vesicular stomatitis virus (VSV) grown in the TMR mutants revealed that the incorporation of radioactive glucosamine was markedly reduced in the mutants. The results indicate that TMR cells are a novel type of membrane mutant.This publication has 39 references indexed in Scilit:
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