Demonstration of Kinetoplast DNA in Dyskinetoplastic Strains of Trypanosoma equiperdum
- 27 February 1976
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 191 (4229) , 858-859
- https://doi.org/10.1126/science.1251198
Abstract
4,6 Diamidino-2-phenylindole (DAPI) forma a highly fluorescent complex with DNA which allows detection of mitochondrial DNA (K-DNA) in normal and dyskinetoplastic strains of Trypanosoma equiperdum. The K-DNA DAPI complexes in the dyskinetoplastic cells, cells lacking detactable K-DNA by other cytochemical methods, are not restricted to a single region of the organism as in the normal strain, but are seen as a row of particles. These observations support the hypothesis that the K-DNA is retained in dyskinetoplastic cells.Keywords
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