A simple method to attach a universal 50-bp GC-clamp to PCR fragments used for mutation analysis by DGGE.
Open Access
- 1 August 1992
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 2 (1) , 83-85
- https://doi.org/10.1101/gr.2.1.83
Abstract
An international, peer-reviewed genome sciences journal featuring outstanding original research that offers novel insights into the biology of all organismsThis publication has 9 references indexed in Scilit:
- Molecular characterization of mild-to-moderate hemophilia A: detection of the mutation in 25 of 29 patients by denaturing gradient gel electrophoresis.Proceedings of the National Academy of Sciences, 1991
- Molecular scanning methods of mutation detection.Journal of Biological Chemistry, 1990
- Comprehensive detection of single base changes in human genomic DNA using denaturing gradient gel electrophoresis and a GC clampGenomics, 1990
- Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.Proceedings of the National Academy of Sciences, 1989
- A variation in the structure of the protein-coding region of the human p53 geneGene, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Tyrosine Kinase Receptor with Extensive Homology to EGF Receptor Shares Chromosomal Location with neu OncogeneScience, 1985
- Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresisNucleic Acids Research, 1985
- DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.Proceedings of the National Academy of Sciences, 1983