Coupling of D2 and D3 dopamine receptors to G‐proteins
- 11 January 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 315 (3) , 223-226
- https://doi.org/10.1016/0014-5793(93)81168-y
Abstract
Recombinant cell lines expressing D2 and D3 dopamine receptor isoforms have been used to study coupling of these receptors to G‐proteins from the effects of GTP to reduce agonist binding affinities in agonist/[3H]spiperone competition experiments. D2(short) expressed in Ltk− cells, D2(long) expressed in Ltk− or CHO cells and D3 expressed in CHO cells all showed coupling to the endogenous G‐proteins of the cells. The detailed agonist binding characteristics indicated that there were differences in receptor/G‐protein coupling for the same receptor (D2(long) expressed in two cell types or for different receptors (D2(long),D2(short) expressed in the same cell type.Keywords
This publication has 7 references indexed in Scilit:
- Structural subtypes of the dopamine D2 receptor are functionally distinct: expression of the cloned D2A and D2B subtypes in a heterologous cell lineMolecular Endocrinology, 1992
- Pharmacology of human dopamine D3 receptor expressed in a mammalian cell line: comparison with D2 receptorEuropean Journal of Pharmacology: Molecular Pharmacology, 1992
- The third dopamine receptor (D3) as a novel target for antipsychoticsBiochemical Pharmacology, 1992
- Molecular biology of dopamine receptorsTrends in Pharmacological Sciences, 1992
- Receptors for neurotransmitters and related substancesCurrent Opinion in Biotechnology, 1991
- Transcription mediated by a cAMP-responsive promoter element is reduced upon activation of dopamine D2 receptors.Proceedings of the National Academy of Sciences, 1991
- Alternative splicing directs the expression of two D2 dopamine receptor isoformsNature, 1989