Studies on the Physiology of a Streptomycin-producing Strain of Streptomyces griseus on Proline Medium

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Abstract
By use of L-proline as the sole N source in a chemically defined medium, yields of 1 g. of streptomycin per liter of medium were obtained in shake flasks. The fermentation cycle was further shortened by combining 0.02% (NH4)2HPO4 with 1% proline. Higher yields were obtained with a 10% inoculum grown on a proline medium than with a comparable inoculum grown on a (NH4)2HPO4 medium. The mechanism of proline utilization differs from processes known to occur with other organisms. S. griseus produces as an intermediate an unidentified amino-N-containing compound when grown or suspended in a proline medium. This intermediate likewise supports streptomycin production.