Solid-phase synthesis of oligodeoxynucleotides containing O6-alkylguanine
- 30 April 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 11 (5) , 811-816
- https://doi.org/10.1093/carcin/11.5.811
Abstract
Practical methods for the routine solid-phase synthesis of oligodeoxynucleotides containing O6-alkylguanine are described. It is shown that if the 2-amino group of the alkylated base is protected with a phenylacetyl group, and a mixture of nitrobenzaldoximate ions and ammonia used to remove the protecting groups from the oligomer at the end of the synthesis, there is negligible formation of 2,6-diainino-purine and that after chromatography pure oligomers are obtained.This publication has 10 references indexed in Scilit:
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