Fluorometric determination of cephalexin, cephradine, and cephatrizine in biological fluids.

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Abstract
Intensely fluorescent products were formed from cephalexin and cephradine in acidic solution containing hydrogen peroxide by hydrolysis at high temperature. A fluorescent product was formed from cephatrizine in solutions containing mercuric chloride by warming at 60.degree. for 50 min after cleavage of the .beta.-lactam ring with 1 N NaOH. Each fluorescent product was readily extracted with ethyl acetate or an acetone-chloroform mixture from neutral solutions and could be partitioned into alkaline solution. The urinary excretion of the antibiotics in human volunteers after oral administration was studied using the new methods.

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