DNA sequence associated with chromosome translocations in mouse plasmacytomas.

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Abstract
A DNA sequence that generates aberrantly rearranged immunoglobulin H chain constant region genes in murine plasmacytomas is shown to participate in a chromosome translocation. This DNA sequence was termed NIARD for non-immunoglobulin-associated rearranging DNA. NIARD rearrangements were found frequently in murine plasmacytomas but were not detected in normal lymphocytes. These rearrangements occasionally involve the switch region of the C.alpha. gene. DNA samples obtained from mouse.sbd.Chinese hamster somatic cell hybrid lines were digested with various restriction endonucleases and analyzed by the Southern transfer technique with a NIARD hybridization probe. These experiments show that NIARD resides on chromosome 15 in the mouse germ line. Since NIARD is found adjacent to the C.alpha. gene (located on chromosome 12) in some plasmacytomas, it is apparent that a translocation involving these 2 chromosomes has occurred. A rcpT(12;15) model was proposed to explain the data. The implications of NIARD rearrangements for malignant transformation are discussed.