The Isolation and some Properties of a Virus-Inhibiting Protein from Phytolacca esculenta

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Abstract
An inhibitor of plant viruses was obtained by fractional ethanol precipitation of the clarified expressed juice of P. esculenta leaves. The material was purified by adsorption on diatomaceous earth (celite) and eluted by 10% NaCl. The inhibitor had an isoelectric point at pH 7, contained 14% N., 8-12% carbohydrate, and is probably a glycoprotein. It was effective in inhibiting infection by tobacco mosaic, tomato bushy stunt, potato X, tobacco necrosis, and cucumber viruses, but had no activity against a Rhizobium bacteriophage. As with previously reported inhibitors of plant viruses, infectivity can be restored by dilution.