Behaviour of Sheep-Immunoglobulin-Bearing and Non-Immunoglobulin-Bearing Lymphocytes Isolated by Nylon Wool Columns

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Abstract
The technique of separating lymphocytes on nylon wool columns has been applied to sheep lymphocytes obtained from efferent lymph. The non-immunoglobulin-bearing (sIg––) lymphocytes which pass through the column were MLR-reactive, responsive only to T cell mitogens, and migrated in vivo like T lymphocytes described in rodents. Immunoglobulin-bearing (sIg+) lymphocytes were MLR-non-reactive, responsive to lipo-polysaccharide and migrated in vivo like B lymphocytes in rodents. It is considered that the majority of sIg–– lymphocytes obtained in this way are T lymphocytes and the majority of sIg+ lymphocytes are B lymphocytes.