Comparison of Ribotyping and Repetitive Extragenic Palindromic-PCR for Identification of Fecal Escherichia coli from Humans and Animals
Open Access
- 1 March 2003
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (3) , 1836-1839
- https://doi.org/10.1128/aem.69.3.1836-1839.2003
Abstract
This report compares the performances of two popular genotypic methods used for tracking the sources of fecal pollution in water, ribotyping and repetitive extragenic palindromic-PCR (rep-PCR). The rep-PCR was more accurate, reproducible, and efficient in associating DNA fingerprints of fecal Escherichia coli with human and animal hosts of origin.Keywords
This publication has 13 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Geographic Variability of Escherichia coli Ribotypes from Animals in Idaho and GeorgiaJournal of Environmental Quality, 2002
- Development of a Procedure for Discriminating among Escherichia coli Isolates from Animal and Human SourcesApplied and Environmental Microbiology, 2002
- Identification of Fecal Escherichia coli from Humans and Animals by RibotypingApplied and Environmental Microbiology, 2001
- A PCR Assay To Discriminate Human and Ruminant Feces on the Basis of Host Differences in Bacteroides-Prevotella Genes Encoding 16S rRNAApplied and Environmental Microbiology, 2000
- Runoff Water Quality from Poultry Litter‐Treated Pasture and Forest SitesJournal of Environmental Quality, 2000
- The distribution of enteric bacteria from Australian mammals: host and geographical effectsMicrobiology, 1999
- Universal ribotyping method using a chemically labelled oligonucleotide probe mixtureResearch in Microbiology, 1997
- Marine waters contaminated with domestic sewage: nonenteric illnesses associated with bather exposure in the United Kingdom.American Journal of Public Health, 1996
- An improved role for faecal coliform to faecal streptococci ratios in the differentiation between human and non-human pollution sourcesWater Research, 1975