The effect of cadmium ions on 2,3‐bisphosphoglycerate in erythrocytes studied with 31P NMR

Abstract
The interaction of cadmium ions with human red blood cell (RBC) 2,3‐bisphosphoglycerate (DPG) was studied by 31P NMR. 31P NMR spectra and 31P T 1 and 31P T 2 relaxation times give evidence for cadmium‐2,3‐bisphosphoglycerate complexation in aqueous solution . 31P NMR spectra indicate the occurrence of a similar complexation in RBC cryolysates. The lag phase (constant polyphosphate level) prior to DPG hydrolysis in incubated red blood cells is lengthened in the presence of cadmium.

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