Postmortem Changes in Binding to the Muscarinic Receptor from Human Cerebral Cortex
- 1 January 1983
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 41 (1) , 102-106
- https://doi.org/10.1111/j.1471-4159.1983.tb11819.x
Abstract
The effects of storage at 4°C on the antagonist and agonist binding properties of the muscarinic acetyl‐choline receptor from fresh surgical and frozen autopsy samples from human cerebral cortex were studied. The number of 1‐[3H]3‐quinuclidinyl benzilate binding sites and their affinities were stable up to 51 h, both when stored as pieces of intact nonfrozen tissue and as a homogenate. The agonist binding properties as measured by the ability of the muscarinic agonist carbachol to compete with l‐[3H]3‐quinuclidinyl benzilate were also stable up to 51 h when the tissue was stored in the form of pieces. The affinity for carbachol decreased when the tissue was stored as a homogenate. The frozen autopsy samples showed no significant differences in binding properties in comparison with fresh neurosurgical tissue.Keywords
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