A Bipartite Late-Budding Domain in Human Immunodeficiency Virus Type 1
Open Access
- 15 November 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (22) , 12373-12377
- https://doi.org/10.1128/jvi.77.22.12373-12377.2003
Abstract
Human immunodeficiency virus type 1 (HIV-1) encodes a PTAP motif within the p6 domain of Gag that recruits Tsg101 and associated factors to facilitate virion budding. In this study, we use trans -complementation assays to demonstrate that the PTAP motif acts synergistically with additional p6 sequences to mediate the formation of infectious extracellular HIV-1 virions. These studies suggest that Tsg101 recruitment is necessary but not sufficient to account for late-budding activity exhibited by HIV-1 p6.Keywords
This publication has 21 references indexed in Scilit:
- Role of ESCRT-I in Retroviral BuddingJournal of Virology, 2003
- Receptor downregulation and multivesicular-body sortingNature Reviews Molecular Cell Biology, 2002
- Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 proteinNature Structural & Molecular Biology, 2002
- Escrt-IIIDevelopmental Cell, 2002
- Endosome-Associated Complex, ESCRT-II, Recruits Transport Machinery for Protein Sorting at the Multivesicular BodyDevelopmental Cell, 2002
- Late Assembly Domain Function Can Exhibit Context Dependence and Involves Ubiquitin Residues Implicated in EndocytosisJournal of Virology, 2002
- The Late Domain of Human Immunodeficiency Virus Type 1 p6 Promotes Virus Release in a Cell Type-Dependent MannerJournal of Virology, 2002
- Tsg101 and the Vacuolar Protein Sorting Pathway Are Essential for HIV-1 BuddingPublished by Elsevier ,2001
- Ubiquitin-Dependent Sorting into the Multivesicular Body Pathway Requires the Function of a Conserved Endosomal Protein Sorting Complex, ESCRT-ICell, 2001
- Ubiquitination of HIV-1 and MuLV GagVirology, 2000