Peritoneal Macrophages from Mice Fed Dietary (n-3) Polyunsaturated Fatty Acids Secrete Low Levels of Prostaglandins
- 1 December 1986
- journal article
- research article
- Published by Elsevier in Journal of Nutrition
- Vol. 116 (12) , 2547-2552
- https://doi.org/10.1093/jn/116.12.2547
Abstract
Peritoneal macrophages from mice fed diets containing 10% menhaden oil incorporated significant amounts of n-3 polyunsaturated fatty acids (n-3 PUFA) into cellular lipids, and this lowered arachidonic acid in these lipid classes compared to those from mice fed diets containing 10% coconut oil. The n-3 PUFA-enriched macrophages secreted significantly smaller amounts of prostaglandin E, thromboxane B and 6-keto-prostaglandin F1α when stimulated with opsonized zymosan or phorbol myristate acetate. These studies demonstrated that menhaden oil-enriched diets influence fatty acid composition and prostaglandin synthesis in macrophages.Keywords
This publication has 17 references indexed in Scilit:
- Dietary fish oil modulates macrophage fatty acids and decreases arthritis susceptibility in mice.The Journal of Experimental Medicine, 1985
- Effect of culture in vitro with eicosatetraenoic (20:4(n − 6)) and eicosapentaenoic (20:5(n − 3)) acids on fatty acid composition, prostaglandin synthesis and chemiluminescence of rat peritoneal macrophagesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1985
- Intracellular calcium does not appear to be essential for arachidonic acid release from stimulated macrophages as shown by studies with Quin-2Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1985
- Biosynthesis of prostanoids, tissue fatty acid composition and thrombotic parameters in rats fed diets enriched with docosahexaenoic (22:6n3) or eicosapentaenoic (20:5n3) acidsThrombosis Research, 1984
- Resting macrophages produce distinct metabolites from exogenous arachidonic acidThe Journal of Experimental Medicine, 1982
- Effect of RNA and protein synthesis inhibitors on the release of inflammatory mediators by macrophages responding to phorbol myristate acetateBiochimica et Biophysica Acta (BBA) - General Subjects, 1980
- Colorimetric determination of phospholipids with ammonium ferrothiocyanateAnalytical Biochemistry, 1980
- A simple, rapid microassay for DNAFEBS Letters, 1975
- Computer program sequence for analysis and summary of radioimmunoassay dataComputers and Biomedical Research, 1972
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959