Fluorogenic Labelling of Carbonylcompounds with 7-Hydrazine-4-nitrobenzo-2-oxa-1,3-diazole (NBD-H)
- 1 April 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 7 (4) , 839-854
- https://doi.org/10.1080/01483918408074006
Abstract
A method for the prechromatographic fluorescence derivatization of carbonyl compounds with 7-hydrazino-4-nitrobenzo-2-oxa-1,3-diazole (NBD-H) is presented. The separation and quantitation of the hydrazones is carried out by TLC and HPLC on silica gel and RP-ma-terials. Detection limits obtained for benzaldehyde by TLC with fluorodensitometric evaluation are 5 ng/spot and by HPLC with fluorescence detection 200 pg.Keywords
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