Mammary Adenocarcinoma (72j) Blood Flow in Mice Treated With ThioTEPA

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Abstract
Tumor (72j mammary adenocarcinoma) blood flow, estimated as the percentage of cardiac output, was measured by the Rb86 method. Mice treated intraperitoneally with 5.0, 2.5, or 1.25 mg/kg of thioTEPA for 6 days or control mice were used. Percent cardiac output correlated well with total tumor weight. The equation, C = C1xb, best expressed the relationship of blood flow to tumor weight (C = % cardiac output per tumor; C1=% cardiac output for 1 g tumor; x = total weight of tumor; b = exponent). There was no significant difference between the b values found with the data from treated or untreated animals. The C values were significantly higher in those animals treated with thioTEPA. Darkening color of the NaOH digest of whole tumors or selected areas correlated with decrease in the estimated cardiac output per gram. The observed (from the literature) cardiac outputs of various species, other than the mouse, were used to provide a least-squares estimate, based on body weight, of the mouse cardiac output (7.2 cc/min). This value was used in estimation of the number of times blood, equivalent to tumor weight, was perfused through tumors of various sizes per day; e.g., 100 mg tumors in mice treated with thioTEPA were perfused with blood equivalent to 1200 times their weight per day, whereas 100 mg tumors from untreated mice were perfused with 700 times their own weight in blood per day. The perfusion rate dropped markedly with increased tumor weight.