Human T gamma globin chain is a variant of A gamma chain (A gamma Sardinia).
- 1 July 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (7) , 3420-3424
- https://doi.org/10.1073/pnas.76.7.3420
Abstract
Isoelectric focusing, cellulose acetate electrophoresis and carboxymethylcellulose chromatography in the presence of Nonidet P-40 allow the separation of pure .gamma. globin chains into 2 fractions. Amino acid analysis of their cyanogen bromide fragment 3 (.gamma.CB3) identifies these fractions as the separated G.gamma.(Gly-136) and A.gamma.(Ala-136) globin chains. Fingerprint and amino acid analyses of the .gamma.Tp9 tryptic peptide from the purified A.gamma. and G.gamma. fractions from 2 different patients demonstrate that the commonly occurring .gamma.Sardinia variant (.gamma. 75 Ile .fwdarw. Thr), also known as T.gamma. chain, has Ala in position 136. The T.gamma. gene is apparently an allele of the A.gamma. locus (A.gamma.Sardinia) rather than a 3rd .gamma. locus.This publication has 17 references indexed in Scilit:
- Abnormal human haemoglobins: I. The comparison of normal human and sickle-cell haemoglobins by “fingerprinting”Published by Elsevier ,2003
- Human globin chain separation by isolectric focusingJournal of Biochemical and Biophysical Methods, 1979
- Gγ and Aγ globin chains separation and quantitation by isoelectric focusingBiochemical and Biophysical Research Communications, 1979
- The chemical heterogeneity of the fetal hemoglobin in normal newborn infants and in adultsMolecular and Cellular Biochemistry, 1977
- THE PRESENT STATUS OF THE HETEROGENEITY OF FETAL HEMOGLOBIN IN β‐THALASSEMIA: AN ATTEMPT TO UNIFY SOME OBSERVATIONS IN THALASSEMIA AND RELATED CONDITIONS*Annals of the New York Academy of Sciences, 1974
- Non-synchronized Suppression of Postnatal Activity in Non-allelic Genes which Synthesize the Gγ Chain in Human Foetal HaemoglobinNature New Biology, 1973
- World-wide Occurrence of Nonallelic Genes for the y-Chain of Human Foetal Haemoglobin in NewbornsNature New Biology, 1972
- Haemoglobin GCopenhagen and haemoglobin JCambridge. Two new β-chain variants of haemoglobin ABiochimica et Biophysica Acta (BBA) - Protein Structure, 1967
- A partial amino acid sequence for sheep haemoblogin ABiochemical Journal, 1967
- An improved method for the fingerprinting of human hemoglobinBiochimica et Biophysica Acta, 1961