DIAGNOSIS OF AVIAN VIRAL DISEASES BY ELECTRON-MICROSCOPY

  • 1 January 1978
    • journal article
    • research article
    • Vol. 39  (3) , 505-508
Abstract
Clinical material from avian species was examined directly by EM for the presence of viruses. Mycoplasma-like and coronavirus-like particles were found in chicken feces. These particles did not appear to be associated with disease and were not propagated in the laboratory. Infectious bursal disease virus was readily detected in impression smears on bursas from experimentally infected birds. Poxviruses were demonstrated in smears made from canarypox lesions. Difficulty in distinguishing intact particles of Newcastle disease virus from mycoplasmas and orthomyxoviruses was resolved by treating viral preparations with deoxycholate. After treatment, Newcastle disease virus was lysed, rendering the nucleocapsid visible, but influenza virus was mainly unaffected. Viral particles that were recognized only with difficulty by direct EM examination were easily more identified using immunoelectron microscopy.

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