Comparisons of Characteristics of Cadmium-Tolerant Bacterium,Pseudomonas aeruginosaG-1 and Its Cadmium-Sensitive Mutant Strain
- 1 April 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 44 (4) , 777-782
- https://doi.org/10.1080/00021369.1980.10864030
Abstract
A cadmium-sensitive mutant strain was isolated from a Cd2+-tolerant bacterium, Pseudomonas aeruginosa G-1 strain by mutagenesis with N-methyl-N′-nitro-N-mtrosoguanidine. The Cd2+-sensitive mutant strain was about 8-times as sensitive to Cd2+ and 2-times as sensitive to Hg2+, Cr2+ and achromycin, and not sensitive to Cu2+, as the parent strain. Vacuole-like substances were observed in the parent strain by transmission electron microscope in the presence of Cd2+, but not in the absence of Cd2+. The mutant strain took up 3.3-times the amount of Cd2+ into cells when compared with the parent strain. Disc electrophoretic patterns showed a characteristic protein band in the parent strain at the stationary phase, but the corresponding protein band was present in the mutant strain at the late exponential phase.This publication has 1 reference indexed in Scilit:
- Cation Transport Alteration Associated with Plasmid-Determined Resistance to Cadmium in Staphylococcus aureusAntimicrobial Agents and Chemotherapy, 1978