Feulgen Staining of Cell Suspensions

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Abstract
A method is described which yields permanent Feulgen preparations of meiotic material from Lilium anthers, eliminating difficulties heretofore encountered. Cells are handled as a suspension through the staining and fixing procedure, and centrifugation and decantation of the supernatant permits changing reagents. Several technics are given which minimize the optical problems created by the heavily sculptured walls of pollen grains, and some applications are suggested.