Differences Among Five Stages ofSchizaphis graminumin Transmission of a Barley Yellow Dwarf Luteovirus

Publisher Website
Google Scholar
Abstract
The duration of each of 4 instars of S. graminum varied from 39 to 48 h at 21.degree. C, intervals that permitted direct comparison among all 5 stages as vectors of the SGV isolate of barley yellow dwarf virus. When aphids of each stage were allowed a 1-day acquisition feeding at 15.degree. C and a 5-day inoculation test feeding at 21.degree. C, percentages of 1st, 2nd, 3rd and 4th instars and adults that transmitted virus were 36, 29, 11, 3 and 2, respectively. Extending inoculation test feedings to 10 days did not change the pattern of stepwise differences in SGV transmission by the 5 stages. Similar large differences also occurred when aphids acquired virus by feeding through stretched Parafilm membranes on concentrated inoculum, or by being injected with SGV. Use of combinations of temperatures from 15.degree. to 30.degree. C did not increase virus transmission by adults. Studies of virus latent periods in the vector suggested that adults were more likely to transmit SGV if they had acquired virus as nymphs. Combinations of long (5-7 days) acquisition feeding periods with long (10 days) inoculation test feedings also resulted in more than occasional transmission by adults. The incremental pattern of virus transmission differences among the 5 stages suggests that the S. graminum-SGV system could be useful for basic studies of virus-vector interactions.

This publication has 0 references indexed in Scilit: