Silicate cement in a cell culture system

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Abstract
Human epithelial cells (NCTG 2544) were grown as monolayer cultures in the presence of silicate cement disks (Bio‐trey 9®). A cytotoxic effect was found on the surface of the disks after 24 h, whereas a corresponding effect was obvious around the disks after incubation for 3 d. In the silicate cement cultures more glucose was utilized and more lactate formed per cell than in control cultures. In the presence of silicate cement, pH of the culture medium decreased during incubation, reaching 6.3–6.4 after 6 d. Phosphate, silicon, zinc, and fluoride were released into the medium from the silicate cement disks. The medium concentration of sodium, however, remained constant, and aluminum was not detected. The concentrations of calcium and magnesium decreased, and experiments with 45Ca showed that calcium was bound to the silicate cement disks.