Effect of Stage of Lactation on Accuracy of Estimating Milk Protein Using Orange G Dye Binding

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Abstract
Dye binding and macro-Kjeldahl methods were compared in determining percent protein in milk during different stages of lactation. Protein was also determined in milk sampled at 12-hour intervals from cows commencing at parturition. Except for colostrum the stage of lactation has no practical effect on accuracy of per cent milk protein determined by dye binding (orange-G).