Rapid determination of bromvalerylurea in biological materials by high-performance liquid chromatography.

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Abstract
A high-performance liquid chromatographic (HPLC) method for the quantitation of bromovaleryl-urea in biological materials is described. A cleanup of the samples for bromovalerylurea was achieved simply and rapidly by using Extrelut column with 1,2-dichloroethane. The detection limit was 5 ng on octadecylsilated silica column with a mobile phase of aceonitrile-10 mM phosphoric acid (22:78) and an ultraviolet detector (225 nm). The calibration curve gave a good linearity over the range of 0.5-50 .mu.g/ml and 20-1000 .mu.g/ml using phenobarbital as internal standard. The method also showed a good reproducibility, the coefficient of variation being 2.0% for blood, 6.8% for urine and 2.7% for brain. The contents of bromovalerylurea in various tissues of a suicide cadaver could be determined with this method. This simple and rapid analytical technique could be useful for the detection of bromovalerylurea from intoxicated subjects in the field of forensic toxicology and emergency medicine.

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