Über das optische Verhalten von Desoxyribonucleinsäure-Histon-Kombinaten

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Abstract
The extinction of varying mixtures of desoxyribonucleic acid (DNA) and original histon at 260 mμ does not show an increase in proportion to the increase in the amount of histon added to the basic, constant DNA concentration, but is characterized by two points of inflexion. The composition of the DNA-histon mixture at the first of these points reveals the N/P quotient (3,7-3,9) characteristic of natural DNA-histons, i. e. a ratio of one basic N atom to 1,5 Ρ atoms. The second point (N/P ratio 8,5-9,5) marks the limit of histon-binding capacity of the complex. Such recombinations of DNA and histon are only incompletely degraded enzymatically by desoxyribonuclease. The rate of degradation is inversely proportional to the histon concentration.

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