MICRO-PIXE ANALYSIS OF FISH OTOLITHS: METHODOLOGY AND EVALUATION OF FIRST RESULTS FOR STOCK DISCRIMINATION

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Abstract
Micro-PIXE has been used to measure the trace element distribution in otoliths from several species of ocean fish, in order to investigate its possible use in stock discrimination. Trace elements detected include Sr, Fe, Mn, Ni, Zn, Cu, Se, Cd, Br, Hg and Pb. Trace elements Na, K, Cl, S and Cl were detected with the electron microprobe. The high sensitivity of PIXE demands a meticulous sample preparation procedure to avoid contamination problems. Practical problems associated with the application of the technique were investigated in detail. Preliminary results indicate that most trace elements except Sr, are present at close to the limits of detection at few ppm, but biologically significant data can be obtained for stock discrimination applications.

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