Two Types of Inclusions in Maize Infected with Maize Stripe Virus

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Abstract
Two types of inclusions were consistently found in the epidermis, mesophyll, vascular parenchyma and phloem elements of maize stripe virus (MStpV)-infected maize (Z. mays) leaves, but not in similar tissues from healthy or maize mosaic virus-infected plants. One type consisted of long, narrow bundles of filamentous, electron-opaque (FEO) material. The bundles were from 30 to 400 nm wide and up to 3.5 .mu.m long and were composed of fine filaments in a paracrystalline array with a periodicity of 3.5-3.7 nm. The FEO inclusions were usually surrounded by a membrane and were found in the cytoplasm and cell vacuole. The other type of inclusion consisted of irregularly shaped masses of amorphous, semielectron-opaque (ASO) material that generally was not enclosed by a membrane. Cells were found that contained both inclusion types. The ASO inclusions were much more abundant in leaves 4-5 wk after inoculation than at 1-2 wk; the incidences of FEO inclusions were similar at both times. Antibodies to the MStpV noncapsid protein bound to the FEO inclusions, antibodies to the MStpV nucleoprotein bound to an unidentified cytoplasmic constituent, and neither antibody bound to ASO inclusions.