Synthesis of Lariat-DNA via the Chemical Ligation of a Dumbbell Complex
- 9 January 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Organic Letters
- Vol. 5 (3) , 273-276
- https://doi.org/10.1021/ol027227e
Abstract
An efficient synthesis of a medium-sized DNA lariat through the chemical ligation of a Y-shaped dumbbell precursor is described. The methodology requires only commercially available phosphoramidites and reagents and affords regioisomerically pure lariat molecules. Characterization of the lariat by Tm analysis reveals that the molecule displays markedly enhanced thermal stability and unimolecular association−dissociation kinetics, consistent with DNA dumbbell behavior.Keywords
This publication has 27 references indexed in Scilit:
- Solid‐Phase Synthesis of Branched OligonucleotidesCurrent Protocols in Nucleic Acid Chemistry, 2002
- Efficient Synthesis of DNA Dumbbells Using Template-Induced Chemical Ligation in Double-Stranded Polynucleotides Closed by Minihairpin FragmentsAntisense and Nucleic Acid Drug Development, 1999
- Regiospecific Solid-Phase Synthesis of Branched Oligonucleotides. Effect of Vicinal 2‘,5‘- (or 2‘,3‘-) and 3‘,5‘-Phosphodiester Linkages on the Formation of Hairpin DNABioconjugate Chemistry, 1997
- Chemical synthesis of oligodeoxynucleotide dumbbellsBiochemistry, 1991
- N-Cyanoimidazole and Diimidazole Imine: Water-Soluble Condensing Agents for the Formation of the Phosphodiester BondNucleosides and Nucleotides, 1989
- Chemical development in the design of oligonucleotide probes for binding to DNA and RNABiochimie, 1988
- Conformational properties of branched RNA fragments in aqueous solutionBiochemistry, 1988
- A dumbbell-shaped, double-hairpin structure of DNA: a thermodynamic investigationBiochemistry, 1987
- Synthesis of branched ribonucleotides related to the mechanism of splicing of eukaryotic messenger RNAJournal of the American Chemical Society, 1985
- Lariat structures are in vivo intermediates in yeast pre-mRNA splicingCell, 1984