Rapid Isolation and Identification of Bacteriophage T4-Encoded Modifications of Escherichia coli RNA Polymerase: A Generic Method to Study Bacteriophage/Host Interactions
- 14 February 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 7 (3) , 1244-1250
- https://doi.org/10.1021/pr070451j
Abstract
Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery.Keywords
This publication has 42 references indexed in Scilit:
- Determining the architectures of macromolecular assembliesNature, 2007
- Mutational Analysis of σ70 Region 4 Needed for Appropriation by the Bacteriophage T4 Transcription Factors AsiA and MotAJournal of Molecular Biology, 2006
- The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: Target proteins identifiedBiochemical and Biophysical Research Communications, 2005
- Novel Protein–Protein Interaction Between Escherichia coli SoxS and the DNA Binding Determinant of the RNA Polymerase α Subunit: SoxS Functions as a Co-sigma Factor and Redeploys RNA Polymerase from UP-element-containing Promoters to SoxS-dependent Promoters during Oxidative StressJournal of Molecular Biology, 2004
- Tethering σ70 to RNA polymerase reveals high in vivo activity of σ factors and σ70-dependent pausing at promoter-distal locationsGenes & Development, 2003
- Translating the Histone CodeScience, 2001
- The Yeast Nuclear Pore ComplexThe Journal of cell biology, 2000
- Inhibition of Escherichia coli RNA polymerase by bacteriophage T4 AsiAJournal of Molecular Biology, 1998
- DNA-binding determinants of the alpha subunit of RNA polymerase: novel DNA-binding domain architecture.Genes & Development, 1996
- Control by bacteriophage T4 of two sequential phosphorylations of the alpha subunit of Escherichia coli RNA polymeraseJournal of Molecular Biology, 1974