Properties of Human “Albumin”: A Metabolic Study of Albumin Extracts from Normal and Diabetic Plasma

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Abstract
" Albumin" prepared from plasma fron normal subjects and from poorly controlled diabetics by a T.C.A.-ethanol extraction procedure strongly stimulates glucose uptake by the isolated rat hemidiaphragm. It is almost certain that this stimulation is due to insulin since the extracts also stimulate glycogen deposition, incorporation of C-14 1-leucine into diaphragm protein and the production of C-14-O2 from I-C-14 glucose by the rat epididymal fat pad; moreover, the glucose-uptake stimulating effect is blocked by a potent insulin neutralizing plasma. The insulin activity can be separated from the albumin by a gel diffusion technic. Albumin itself is quite inert It was not possible to demonstrate insulin antagonism by the extracts in this study.