Genetic evidence for the existence of a repressor that modulates colicin D expression on plasmid ColD-CA23
- 1 July 1988
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 213 (1) , 36-41
- https://doi.org/10.1007/bf00333395
Abstract
The plasmid ColD-CA23, a high copy number plasmid of 5.12 kb, contains genes for colicin D (cda), for immunity colicin D (cdi), and for a lysis function (cdl). These genes are arranged on a contiguous 2.4 kb fragment in the following sequence: cda, cdi, cdl. They are transcribed in two operons, one transcribing cda and cdl from a SOS inducible promoter, the other transcribing cdi in the opposite direction. The expression of cda and cdl is modulated by a repressor, cdr, which is encoded on the same transcript as cda and cdl. In the absence of this repressor, transcription from the SOS inducible colicin D promoter is exceptionally strong and leads to protein contents up to 50% of total cellular proteins. This autoregulative repressor is a new finding in the control mechanisms of expression of colicins. We have also identified the gene product of cdl to be a 10000 dalton protein.This publication has 18 references indexed in Scilit:
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