Determination of Sulfaquinoxaline, Sulfathiazole, Sulfamerazine, and Sulfamethazine in Feed Concentrates or Premixes
Open Access
- 1 November 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 56 (6) , 1475-1479
- https://doi.org/10.1093/jaoac/56.6.1475
Abstract
Sulfaquinoxaline is determined by its UV absorbance at about 358 nm, where the other 3 sulfonamides do not absorb. Sulfathiazole, sulfamerazine, and sulfamethazine are determined by a quantitative TLC procedure, based on the separation of the compounds on silica gel plates; the spots are extracted and the centrifuged extracts are analyzed spectro-photometrically. A method of calculating the total sulfonamide content, independent of the individual components, is also introduced.Keywords
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