CONJUGAL PAIRING IN ESCHERICHIA COLI

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Abstract
Conjugating pairs of cells were found in crosses of highly fertile strains of E. coli. Pairs (279) were isolated with micromanipulation and clones established from each exconjugant. The Hfr mating type exconjugant was usually viable, but none of 222 clones contained recombinants. Sixty-six of 190 exconjugants of the F mating type contained recombinant as well as parental types. The recombinant types were strongly biased toward the F parental type. While genetic recombination is thus correlated with conjugation, and probably results from the transfer of a nucleus from one multinucleate Hfr cell to the other F mate, cytological details are still indecisive.

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