Studies of sebum. 4. The estimation of squalene in sebum and sebum-like materials
- 1 November 1953
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 55 (4) , 637-640
- https://doi.org/10.1042/bj0550637
Abstract
A method of estimating unsaturated hydrocarbons is descr. which is applicable to small amts. (5 mg.) of sebum and similar materials, and which gives an avg. recovery of 80% of added squalene. After saponification, a hydrocarbon fraction is isolated chromatographically and the unsaturated material therein is detd. iodometrically. The method may be used for the detn. of squalene in sebum and other lipids provided that the presence of squalene is first established and that no other unsaturated hydrocarbons are present Data on the Liebermann-Burchard reaction of squalene are recorded.Keywords
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