Reversible heat injury in the marine psychrophilic bacterium Vibrio marinus MP-1

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Abstract
A modification of the double-medium technique of Iandolo and Ordal (1966) was used to study heat injury in the obligately psychrophilic marine bacterium Vibrio marinus MP-1. Results using this technique demonstrated that heated cell suspensions contained a mixed population of uninjured, reversibly injured, and irreversibly injured (killed) cells. The age of the culture, temperature, and length of heating time influenced the ratio in the population of the various types of cells. The repair process of reversibly injured cells was affected by the surface area-to-volume ratio of the medium used to support repair.

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