Semimicro Method for Determination of Serum Uric Acid Using EDTA—Hydrazine

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Abstract
A semimicro method is described for the determination of uric acid by manual and automated procedures. Serum uric acid reduces phosphotungstic acid in an alkaline medium of EDTA sodium tungstate and hydrazine sulfate, the latter being used as a color intensifier. The use of sodium tungstate eliminates the development of interfering turbidity encountered with other alkalizing reagents. This method requires only 0.2 ml. of serum. The presence of ascorbic acid, sodium salicylate, cysteine, and/or glucose does not interfere. A linear relationship is obtained upon comparing this method with the sodium carbonate and the differential ultraviolet spectrophotometric methods.

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