REVERSIBLE INHIBITION OF NORMAL HUMAN PROKERATINOCYTE PROLIFERATION BY TYPE-BETA TRANSFORMING GROWTH FACTOR-GROWTH INHIBITOR IN SERUM-FREE MEDIUM
- 1 April 1986
- journal article
- research article
- Vol. 46 (4) , 2068-2071
Abstract
Type .beta. transforming growth factor-growth inhibitor (TGF.beta./GI) causes normal human prokeratinocytes to arrest growth predominantly in the G1 phase of the cell cycle within 48 h after log phase cultures are exposed to the factor in serum-free medium. The growth arrest induced by TGF.beta./GI is reversible because the cells from treated cultures can be replated into fresh medium and grown into large colonies. Normal prokeratinocytes are demonstrated to secrete TGF.beta./GI-like molecules into the culture medium and to have specific cell surface receptors for this molecule. In contrast, a human squamous cell carcinoma, SCC-25, does not arrest growth when exposed to TGF.beta./GI. These cells, unlike the normal prokeratinocytes, do not exhibit detectable cell surface receptors for the factor.This publication has 2 references indexed in Scilit:
- Specific binding to cultured cells of 125I-labeled type beta transforming growth factor from human platelets.Proceedings of the National Academy of Sciences, 1984
- Mouse embryos contain polypeptide growth factor(s) capable of inducing a reversible neoplastic phenotype in nontransformed cells in cultureJournal of Cellular Physiology, 1982