Evidence for Metabotropic Excitatory Amino Acid Receptor Heterogeneity: Developmental and Brain Regional Studies
- 1 July 1992
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 59 (1) , 252-258
- https://doi.org/10.1111/j.1471-4159.1992.tb08898.x
Abstract
To examine whether multiple subtypes of the excitatory amino acid (EAA) receptor coupled to phosphoino‐sitide (PPI) hydrolysis exist, we have pharmacologically characterized the PPI response in neonatal and adult rat brain. Activation of PPI hydrolysis was determined by the accumulation of [3H]inositol monophosphate in brain slices prelabeled with [3H]inositol. In neonatal hippocampus, D,L‐2‐amino‐3‐phosphonopropionic acid (AP3; 1 mM) inhibited the m‐1‐aminocyclopentane‐1,3‐dicarboxylic acid (IUPAC nomenclature; ACPD; 100 μM)‐ and quisqualate (Quis; 100 μM)‐stimulated PPI hydrolysis by 73 and 66%, respectively, but had no effect in neonatal cerebellum. In adult hippocampus, AP3 stimulated PPI hydrolysis with potency and efficacy comparable to those of Quis and ACPD and completely masked the Quis concentration‐response curve. In adult cerebellum, only Quis behaved as a full agonist on the PPI response. The Quis concentration‐response curve was shifted rightward with a fourfold decrease in potency in the presence of ACPD (5 mM), whereas it was nearly additive with the PPI response induced by AP3 (5 mM). Thus, our data reveal significant developmental and brain regional differences in metabotropic EAA receptor responses and support the notion that this receptor is heterogeneous, in both a regionally specific and a develop‐mentally dependent manner.Keywords
This publication has 26 references indexed in Scilit:
- Intracellular Ca2+ activates phospholipase CPublished by Elsevier ,2003
- Sequence and expression of a metabotropic glutamate receptorNature, 1991
- The release and uptake of excitatory amino acidsTrends in Pharmacological Sciences, 1990
- Selective activation of phosphoinositide hydrolysis by a rigid analogue of glutamateNeuroscience Letters, 1990
- Inositolphospholipid-linked glutamate receptors mediate cerebellar parallel-fiber-Purkinje-cell synaptic transmission.Proceedings of the National Academy of Sciences, 1989
- The Excitatory Amino Acid Receptors: Their Classes, Pharmacology, And Distinct Properties In The Function Of The Central Nervous SystemAnnual Review of Pharmacology and Toxicology, 1989
- Two classes of N-methyl-D-aspartate recognition sites: differential distribution and differential regulation by glycine.Proceedings of the National Academy of Sciences, 1988
- Assessment of rat brain alpha1-adrenoceptor binding and activation of inositol phospholipid turnover following chronic imipramine treatmentNeurochemical Research, 1988
- Excitatory amino acid recognition sites coupled with inositol phospholipid metabolism: developmental changes and interaction with alpha 1-adrenoceptors.Proceedings of the National Academy of Sciences, 1986
- Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositidesBiochemical Journal, 1983