Studies on Phosphoproteins from Fish Eggs

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Abstract
1. A phosphoprotein containing 10.6% of phosphorus has been isolated from the eggs of Pacific herring by means of phenol extraction and DEAE-cellulose column chromatography. 2. Only 8 kinds of amino acid including a small amount each of the 2 kinds were detectable in the acid hydrolysate of the phosphoprotein. The protein contained only serine as hydroxyl group-bearing amino acid and this amino acid occupied more than 2/3 of total amino acid residues. No amino sugar was detectable. 3. A molecular weight of 4, 200±400 was calculated from the sedimentation equilibrium data for the phosphoprotein. The following formula has been given tentatively for the protein on the basis of the molecular weight: (Lys)1(Arg)1(Asp)1(Phe)1(Leu)3(Ser)23–25(Phosphate)16. 4. The results of an alkali-catalyzed β-elimination reaction suggested that all the phosphorus in the phosphoprotein are present in the form of monoesters linked to the hydroxyl groups of serine. 5. Two moles of DNP-group were introduced per 4, 200 g of the protein. The N-terminal residue of the major component of the preparation was determined to be lysine by the analysis of DNP-products.

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