Comparative Mycelial Protein and Enzyme Patterns In Four Species of Ceratocystis

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Abstract
SUMMARY Mycelial protein and alkaline phosphatase complements from 13 isolates of Ceratocystis cocrulescens, C. fagacearum, C. fimbriata and C. ulmi were extracted from acetone powders, separated on Polyacrylamide gels by disc electrophoresis and stained. The general protein profile revealed a minimum of 10 and a maximum of 15 separate proteins. Greater similarity in protein composition was observed within than among isolates of the species, although 5 bands were common to all 4 species. Identical alkaline phosphatase patterns were observed within each species, but there was no alkaline phosphatase site common to all. C. fimbriata and C. ulmi shared 3 alkaline phosphatase sites, while C. coerulescens and C. fagacearum shared 2. These findings provide additional taxonomic criteria, and support the current separation of these species. They further demonstrate inter- and intra-specific biochemical differences and similarities in forms with known perfect stages, thereby expanding our concept on intra- and inter-specific biochemical variation which exists.